Morgan State testing tool could predict future sewer spills, researcher says

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A unique circular DNA molecule found in human feces can be used to monitor leaks from aging sewer infrastructure and predict future collapses, such as the one that contaminated the Potomac River in January, researchers at Morgan State University say.

“If you want to find out if it’s coming from a human source, you can target this marker,” said Samendra Sherchan, professor of environmental biotechnology and environmental health sciences at Morgan State. “This new molecular test is highly efficient and cost-effective compared to lab culture tests,” which measure which bacteria grow over time from a sample.

His work focuses on a specific plasmid, a circular strand of DNA found commonly in human digestive tracts that bacteria use as a sort of biological survival toolkit. The plasmid, known as pBI143, is common in human digestive systems, but not found in waste from birds or animals.

Sherchan said the technology for identifying this molecule returns results in less than a day, compared to 24-hours for Environmental Protection Agency-mandated bacterial culture tests used to validate the safety of beaches and waterways for recreation. The testing is also similar to near-real-time monitoring technology installed in the Baltimore Inner Harbor in April, he added, though that technology does not identify the source of the bacteria.

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